Enzyme lab manual

This laboratory manual will accompany you during your laboratory for Biology I. 8 Enzymes. Enzymes are macromolecular biological catalysts. The molecules upon which enzymes may act are called substrates and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme. This Biochemistry laboratory seeks to model work performed in a biochemical research laboratory. The course will guide you through basic lab techniques, investigations into DNA and enzyme kinetics, an intensive purification and characterization of an unreported protein. 1. Pour 5ml of substrate, 0ml of enzyme (no enzyme for negative control), and.5ml of water into test tube A1. 2. Seal test tube A1 with a stopper immediately after the enzyme is added. 3. Record the reaction rate using the Logger Pro software. 4. Repeat steps using the new measurements as described in table 1. Table 1 Substrate Enzyme Water Total.

1. Pour 5ml of substrate, 0ml of enzyme (no enzyme for negative control), and.5ml of water into test tube A1. 2. Seal test tube A1 with a stopper immediately after the enzyme is added. 3. Record the reaction rate using the Logger Pro software. 4. Repeat steps using the new measurements as described in table 1. Table 1 Substrate Enzyme Water Total. The enzyme is found in high concentrations in a organelle in cells called the peroxisome. One of the functions of catalase is to prevent a toxic accumulation of hydrogen peroxide (H2O2) in cells. It catalyses the conversion of hydrogen peroxide to water and molecular oxygen. Hydrogen peroxide is a by product of metabolic processes. This Biochemistry laboratory seeks to model work performed in a biochemical research laboratory. The course will guide you through basic lab techniques, investigations into DNA and enzyme kinetics, an intensive purification and characterization of an unreported protein.

Biology I Lab instructions: Enzymes Lab. Metabolism • All of the chemical reactions occurring in a cell • Enzymes increase the rate Enzyme Terms. Enzyme Activity - Distance Learning Lab Many lab activities can be conducted with our Wireless, PASPORT, Essential Biology Teacher Lab Manual. proposed for Enzyme Catalysis (AP Bio Lab #2, Biology Lab Manual). that measure the rate of enzyme activity (i.e. Gen Nelson, Catalase Lab.